Jess from ProteinSimple, a Bio-Techne brand, automatizes this protein separation & immunodetection from traditional Western dabbing. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary. Set up, leave and remotely review your data in just 3 hours! Just load your samples and reagents into the microplate, and Jess does the rest. Formaldehyde fixation, C Fox, F Johnson, J Whiting and P Roller, Journal of Histochemistry and Cytochemistry, 1985; 33:845-853. This protocol also describes treatment of glycosylated proteins with PNGase F. Dilute the antibodies in Antibody Diluent 2. Multiple with chemluminescence & incandescence canal. The variability in the manual processes that impact reproducibility, quantitation, time to result, and overall data reliability is eliminated. PDF Related Consumables - WES protein simple system vs traditional Western blotting? Plus, use the Stellar Total Protein assay to normalize your multiplexed results to total protein abundance within the same lane. It takes away so much of the pipetting and manipulation error. Jess' same-time detection also gives you more consistent, reliable results than traditional assays without having to stain and wash a single blot! User Guide for Wes P/N 031-108 Revision 7, July 2018 For research use only. Go further with high sensitivitybest in class fluorescence detection gets all the information you need in one shot. Frontiers | Methodologies to unlock the molecular expression and Pop any bubbles observed in the Separation Matrix wells with a pipette tip. Please note this module can only be used with Jess. Come back to fully analyzed and quantitated results in just 3 hours. Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch, "We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. Sample preparation, separation, and immobilization for theChemiluminescent Total Protein Assayare exactly the same asSimple Western automated immunoassays. ", Yotis Senis, Ph.D., Simple Western data are displayed by lane in a virtual blot-like image similar to traditional Western blot results. Just load your samples and reagents into the microplate . "Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform. User Guide for Jess Customer Service and Technical Support Customer Service and Technical Support Telephone (408) 510-5500 (888) 607-9692 (toll-free in the US and Canada) Fax (408) 510-5599 E-mail support@proteinsimple.com Web www.proteinsimple.com Address ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 USA User Guide for Jess page 33 . Get the picture with Jesss blot imaging system. Or add total protein without giving up a detection channel. ", Nicolas G. Bazan, MD, PhD, Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. With a few clicks, you'll be analyzing immunoassay like standard curves and precisely quantifying your protein. Copyright 2023 Bio-Techne. Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University, "The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. ACD branded products will be available on biotechne.com in the near future. Jess automated western blot systemshes like multiplexed Western blot meets ELISA in one. By continuing to use this website you acknowledge this and agree to our cookie policy. With Stellar modules, low abundance proteins can be detected, and multiple targets can be multiplexed in the same sample using multi-color fluorescence channels. Chemiluminescent Total Protein Detection on Simple Western. Which Brands are Currently Available on biotechne.com? Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. 18 Share 2.8K views 2 years ago Watch this video to learn about WES and JESS - two highly reproducible, capillary based, automated western blot instruments by ProteinSimple. <>/Metadata 248 0 R/ViewerPreferences 249 0 R>> NC1839594. Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. Protein Simple- Jess. Jess processes up to 25 samples in three hours, and Peggy Sue processes up to 96 samples overnight. FAX 612 656 4400. Learn more about Jess by ProteinSimple, a Bio-Techne brand, and western blotting by visiting the Jess instrument page. ", Dr. Mattia Volta, x][SH~'Pbo4BuJ}&&,gzyc,[4,I]%&}6b-YYyEU^o'WlM9Ulz|pY1]M?$}x/>TI Insert a capillary cartridge into the cartridge holder. The appropriate control and low variability between plates using Simple Western means we can complete our studies in weeks rather than months. Jess gives you four ways to analyze proteins. Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. I need to do protein normalization Minneapolis, MN 55413 5. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Privacy Policy. Like multiplexed chemiluminescent and fluorescent assays on the same sample at the same time. Learn more about this offer atSimple Western Antibody Submission Promotion. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods. Has anyone used the ProteinSimple Wes capillary western - ResearchGate For more information visit or contact us at: Toll-free: 888 607 9692 Tel: 408 510 5500 . Assistant Professor, Womens and Childrens Health, Karolinska Institute, Stockholm, Sweden. Minneapolis, MN 55413 ", Melyssa Bratton, Ph.D., It would take about a week to run 40 proteins using Western blotting. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, View all Automated Western Blot Instrumentation FAQs. Download the Total Protein Assay for AAV Analysis App Note to learn more about the use of ultra-high sensitivity total protein assays for AAV analysis in the development of cell & gene therapies. Stop, analyze and wow! After separation & immobilization of your sample proteins, the Protein Normalization Assay Module for Simple Western uses a proprietary molecule to fluorescently label proteins in your sample for detection. Best of all, Jess's florescent detection capabilities enable two color protein detection for multiplexing on top of protein normalization. Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples. ProteinSimple RePlex Module for Jess - Fisher Sci Working with low abundance targets or precious samples? Department of Immunology, Landspitali, the National University Hospital Iceland, "Our lab came across ProteinSimples Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables. Just load your samples and reagents into the microplate and Jess does the rest. YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - ProteinSimple - MedicalExpo Using 5 times more concentrated Biotin Labeling Reagent, total protein detection using Simple Western can surpass the sensitivity of SDS-PAGE total protein stains including SYPRO Ruby. Protein analysis comes with many challenges, labor intensive protocols, increase time to result, and multiple hands-on steps increase user error and data variability. PDF Compass Software for Simple Western Size Assays Quick Reference Guide Just load your samples and reagents into the microplate, and Jess does the rest. Copyright 2023 Bio-Techne. with Jess or Wes, and up to 96 samples with Sally Sue or Peggy Sue, all in a fully automated fashion and with a built-in total protein assay that is unaffected by reducing agents like -ME. Store the unopened product at room temperature. ACD branded products will be available on biotechne.com in the near future. With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process. This new feature, called RePlex removes the antibodies from the first round of probing to perform either a second round of probing with new antibodies or total protein detection. Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. Stellar NIR / IR modules for Jess set the standard for fluorescence Western blotting detection sensitivity. ", Brian Perrino, Ph.D., We offer a 100% guarantee on all our products. Open Compass software v6.0 or higher (Jess, Abby, or Wes) or Compass 5.0 (NanoPro 1000, Peggy Sue, Sally Sue) and click on the Assay icon. Associate Professor, Department of Genetics and Genome Sciences, UConn Health, "Weve been able to increase productivity dramatically with Wes. Let your sample go further with Jess. LOD Comparison of Stellar NIR / IR, Chemiluminescence, and Leading Competitor for Fluorescence Western Blotting using RNase A as the target (Learn more). Protein analysis comes with many challengeslabor-intensive protocols increase time to result and multiple hands-on steps increase . Best of all, the transition is simplethe same antibodies you use for Western blotting protocols can be used with Simple Western assays. For chemiluminescent detection, samples are first incubated with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. By continuing to use this website you acknowledge this and agree to our cookie policy. With Jess, get industry-leading sensitivity in chemiluminescence and fluorescence channels and the advantage of flexible multiplex analysis.